The Transformation System of Oil Sunflower(Helianthus annuus L.)
Jinghui Yang · Northern Horticulture · 2010
In order to establish the system of genetic transformation on oil soybean by Agrobacterium medium transformation of Agrobacterium tumefaciens strains C58C1 with pCB302 vector,the concentration of herbicide,the selection of explants and pre-culture were researched.The results showed that more suitable concentration of herbicide was 3 mg/L,the more desirable explants were stems,the more desirable time of pre-culture was 7 days with highest transformation ratio.