Influences of hesperidin on the regulation of serum lipids levels by simvastat in and CYP4503A mRNA expression
Lu Hong · Zhongguo yaolixue tongbao · 2004
AIMTo study the influences of hesperidin on the regula tion of serum lipids levels by simvastatin and its mechanism. METHODS Wistar rats were divided randomly into five groups:control group,model g roup,simvastatin group(SV group), low dose hesperidin +SV group(LDHS group) and high dose hesperidin+SV group(HDHS group).The control group were fed with common diet. The model group were fed with high lipid diet. Other groups were fed with diet containing high lipid,5 mg·kg -1 SV and different doses of hesperid in accordingly. After 8 experimental weeks,the serum lipids levels and CYP4503Am RNA expression in livers and small bowels of the rats were detected. RE SULTSAfter 8 weeks of high lipid diet, the levels of TC,TG and LDL-C in model groups increased significantly (P0 01).The levels of TC,TG and LDL -C in simvastatin groups decreased significantly versus model groups(P0 01 or P0 05). The levels of TC,TG and LDL-C in groups fed with hesperidi n decreased in a dose-dependent manner versus the groups fed with simvastatin a lone. The levels of CYP4503A mRNA expression had no significant changes in the l iver,but significantly increased in the small bowel of model groups v ersus control groups. The expressions were found to be higher in liver, and were sig nificantly higher in small bowel of simvastatin group than that of model groups. When hesperidin were added, the levels of CYP4503A mRNA in liver and small bowe l had a decreasing tendency and significantly decreased in HDHS group(P0 05). CONCLUSIONHesperidin enhances the regulating effects of sim vastatin on serum lipids profile. The mechanisms might be related to its effect on suppressing CYP4503A mRNA expression in livers and small bowel.