Clone and Expression of a Novel Copper/Zinc-Superoxide Dismutase from Cervus nippon Temminck
Xiuyun Ye · Biotechnology(Faisalabad) · 2010
Objective:A gene encoding a novel copper/zinc-superoxide dismutase ( Cu,Zn-SOD) from Cervus nippon Temminck was cloned and expressed in E.coli Rosetta( DE3) .Method:By using the method of RT-PCR and PCR,the gene was cloned from the liver cells of Cervus nippon Temminck and fused with the vector pET21a(+) .After the new vector pET21a(+)-SOD had been transformed into Rosetta ( DE3) ,the recombinant Rosetta( DE3) was induced by IPTG.Result:The results showed that a copper/zinc-superoxide dismutase from Cervus nippon Temminck could be successfully expressed with activity 257U/ml.Conclusion:The Cu ,Zn-SOD of Cervus nippon Temminck with enzyme activity was successfully expressed in E.coli Rosetta( DE3) .