Regulation of effective components in Equisetum on apoptosis-related genes in cultured endothelial cells induced by ox-LDL
Zhi Li · Zhongguo laonianxue zazhi · 2012
Objective To extract the effective components in Equisetum,observe its effect on Caspase-3,Bax,Bcl-2 mRNA expression in cultured human umbilical vein endothelial cells(HUVECs) induced by oxidized low density lipoprotein(ox-LDL) and discuss the mechanism of protective vascular endothelium and anti-atherosclerosis(AS).Methods Equisetum was extracted by ethanol.After filtrating it,the macro porous resin was used to isolate and purify the Equisetum extract.The content of Quercetin and ferulic acid in extract were determined by ultraviolet absorption spectrophotometer.HUVECs were cultured in vitro and divided into three groups,then stimulated by ox-LDL(60 μg/ml).The expressions of Caspase-3,Bax,Bcl-2 mRNA were assayed by RT-PCR.Results The total Quercetin average level was 1.69 mg/g,the total ferulic acid average level was 1.23 mg/g.The purity of Equisetum effective components was 63.28%.Compared with the model group,the expression of Caspase-3 and Bax mRNA in Equisetum effective components group were significantly lower(P0.01),the expression of Bcl-2 mRNA was significantly increased(P0.01),Bax/Bcl-2 ratio evident lower(P0.01).Conclusions The purity of the extract reaches the standards as effective components.The flavonoids and phenolic acids in Equisetum may play an important role in inhibition of apoptosis through regulating the apoptosis-related genes Caspase-3,Bax,Bcl-2 in HUVECs induced by ox-LDL.