Effects of Atorvastatin on aging-related fator level in myocardium of aging rats
Ping Ye · China Medical Herald · 2013
Objective To investigate the changes of aging-related fators' level such as malondialdehyde,lipofuscin and β-galactosidase in myocardium of aging rats and the effects of Atorvastatin on them.Methods 90 Wistar rats at 20months old were divided into three groups with 30 per group: aging control group,high dose Atorvastatin group [10 mg/(kg.d)],low dose Atorvastatin group [1 mg/(kg.d)].Another 30 Wistar rats at 3-months old were as young control group.Atorvastatin was delivered by intragastric administration for 4 months.Normal sodium was delivered to control groups by intragastric administration too.After 4 months of intragastric administration,the myocardium sample were got and malondialdehyde,lipofuscin,β-galactosidase were detected by kit.Results ①Aging-related fators′level in young control group were as below: malondialdehyde(50.9±11.1)(nmol/mg)/prot,lipofuscin(326.7±39.5)(U/mg)/prot,β-galactosidase(2.1 ±0.3) number/HP.Aging-related fators' level in aging control group were as below: malondialdehyde was(98.5±16.3)(nmol/mg)/prot,lipofuscin was(504.8 ±40.3)(U/mg)/prot,β-galactosidase was(51.9±13.7) number/HP.The malondialdehyde,lipofuscin and β-galactosidase content in the aging rats were significantly higher than those of young rats(P 0.01).②Aging-related fators' level in low dose Atorvastatin group were as below: malondialdehyde was(77.4 ± 14.0)(nmol/mg)/prot,lipofuscin was(428.1 ±33.5)(U/mg)/prot,β-galactosidase was(37.9±12.9) number/HP.Aging-related fators′level in high dose Atorvastatin group were as below: malondialdehyde was(64.3 ±15.8)(nmol/mg)/prot,lipofuscin was(394.1±29.6)(U/mg)/prot,β-galactosidase was(28.3±8.8) number/HP.The malondialdehyde,lipofuscin and β-galactosidase in both two dose Atorvastatin groups were significantly lower than those of aging control group(P 0.01),and high dose Atovastatin groups showed a lower level than low dose group(P 0.01 or P 0.05).Conclusion Aging-related fators′level such as malondialdehyde,lipofuscin and β-galactosidase in myocardium of aging rars were significantly higher than those of young rats,and Atorvastatin treatment can inhibit the expression of these factors.