Spectrophotometric Study on the Interaction Between Picramazochrom and Serum Proteins
Ma Wei · Huaxue Shijie (Chemical World) · 2003
It was found that Picramazochrom can react with proteins forming a violet complex in the Clark Lubs medium at pH1.2 and room temperature, which gives the absorption peak at 625 nm with 100 nm of bathochromic shift in from that of Picramazochrom. A molar absorptivity e 625 2.728×10 5 L/(mol·cm) and linearity range of 10~150 mg/L for BSA were determined. The optimum reaction conditions were investigated, and a new method for the determination of proteins was developed based on this binding reaction. The proposed method, which is easy, rapid, stable, and of good selectivity and high sensitivity, has been used for the determination of total proteins in human serum samples . The results obtained by this method were agreed with those obtained by the Coomassie brillant blue G 250 method.