The Effect of Dhcr24 on Blood Chemistry in the Dhcr24 Transgenic Mouse
Lianfeng Zhang · Zhongguo bijiao yixue zazhi · 2010
Objective To establish the transgenic mouse of 24-dehydrocholesterol reductase gene(Dhcr24) to study the effect of Dhcr24 on metabolism in vivo.Method The mouse Dhcr24 was clone by RT-PCR and the sequence of the cDNA was confirmed by sequence analysis.The transgenic plasmid was constructed by inserting the Dhcr24 gene in the downstream of CMV promoter.The transgenic mice were generated by microinjection method and the genotype was detected by PCR.The expression levels of the gene were determined with RT-PCR and Western Blot.The blood biochemistry items were detected with a Photometer Reflotron Plus for blood following clinical procedure.Result Two transgenic C57BL/6J lines of Dhcr24 gene with different expression levels were established.The expression of the Dhcr24 gene was obviously detected in the liver and spleen.The transgenic mice showed obviously decreased levels of weight,lactic dehydrogenase(LDH),total protein(TP),Albumin(Alb) and serum creatinine(SCr) compared with that of the wild type mice,while the increased high-density lipoprotein cholesterol and increased alkaline phosphatase(ALP) were detected in the transgenic mice.Conclusion The DHCR24 transgenic mice were established successfully and indicated the Dhcr24 gene regulated body weight of the female mice and the blood chemistry included the levels of LDH,TP,Alb,SCr,HDL-c and ALP of female mice.