Protection of Spinal Cord Ischemic Injury by Means of Regional Infusion with Hypothermic Normal Saline and Adenosine
Xiao Rong · Zhongguo xiong-xin xueguan waike linchuang zazhi · 2001
Objective To study the protection of spinal cord ischemic injury by means of regional infusion with hypothermic normal saline and Methods Thirty New Zealand adult rabbits were randomly divided into three groups, group A( n =10): ischemic group, group B( n =10):hypothermic normal saline regional infusion, and group C( n =10):hypothermic normal saline infusion with adenosine. The experimental model was established in rabbits through cross clamping of abdominal aorta inferior to the left renal artery. Hemodynamic data, malondialdehyde and superoxide dismutase of spinal cord were measured in every group. Spinal cord function was assessed by the Tarlov Scale postoperatively and spinal cords were harvested for histologic analysis. Results There was no marked differences in blood pressure and heart rate among three groups. The malondialdehyde increased and superoxide dismutase decreased in group A, the change was small in group B and group C. Rabbits in group A became paraplegic while group B were partly restored and group C were fully restored to normal. Histologic examination of spinal cords from group B and group C revealed no obvious evidence of ischemic injury, whereas group A showed extensive spinal cord injury with central gray coagulative necrosis, macrophage infiltration, dissolution of Nissl substance. Conclusion Regional infusion with hypothermic normal saline and adenosine can protect the spinal cord ischemic injury distinctly. Adenosine can not only decrease adenosine triphosphate depletion of ischemic tissue, but also has strong neural protective function.