The Evaluation of Differential Count by Beckman Coulter LH 750 and its Application
Yuze Huang · Journal of Practical Medical Techniques · 2004
Objectives To evaluate the accuracy of the nucleated cells count w hich including differential count by Coulter LH 750. Methods Comp ared the repea tedly of differential count by Coulter LH 750 and Coulter MAXM. Counted eosinop hil and mononuclear and differential count of 30 blood samples by microscope, Ob served the correlation of differential count by microscope and Coulter LH 750 by statistics analysis. When have clue of erythroblast cells by Coulter LH 750,id entified it by microscope examination. Results The repeatedly of C oulter LH 750 is higher than Coulter MAXM. There were perfect correlation of differential coun t between by microscope count and LH 750, and the correlation of eosinophil and mononuclear count had raised (r=0.925 and 0.864), the coincidence ratio of eryth roblast was 87.5 %. The mass of clue of abnormal nucleated cells by LH 750 can b e en identified by microscope examination. Conclusions The accuracy of differenti al count by LH 750 is higher than others Coulter blood cell analysor. LH 750 is effective in screening abnormal nucleated cells, and it must been identified by microscope examination.