Grain-size Selection of Nanogold Particles in Liquid Immunoassay and Its Application
Na Wang · Journal of Heze Medical College · 2010
Objective To explore the effects of nanogold in different sizes on liquid immunoassay system.Methods The gold nanoparticles in sizes of 10,21,50 nm were prepared to label rabbit hCG antiserum,selecting spectrophotometry and resonance resonance scattering spectrometry to analyse The nanogold-labeled antiscruni and immunonogold-complex..Results The labeling efficiency of nanogold in size of 10 nm was better,with good linear range.The nanogold-labeled antiserum was used to assayed hCG in urine samples,it has satisfactory results.Conclusion It's better to choose the nanogold particles in sizes of 8～15 nm in liquid immunoassay by resonance resonance scattering spectrometry.