Evaluation of quantitative detection of plasma D-dimer with gold colloids immunofiltration
Xiaoguang Xing, Yang Chen · Tianjin Yike Daxue xuebao · 2004
Objective:To evaluate quantitative detection of plasma D-dimer with gold colloids immunofilˉtration(GIA)in comparison with ELISA.Methods:Use ELISA and GIA at the same time to detect plasma D-dimer,and compared the results.Results:When cut-off value was0.30mg/L,sensitivity and specificity of ELISA and GIA respectively were90.7%、94.5%and92.5%、96.2%.paired t-test of the two methods showed no significant difference(P0.05).According to NCCLS EP9-A,the results of linear regression and correlaˉtion of GIA and ELISA showed good relationship(r=0.9966).Conclusion:GIA has not only the advantageous analytical property of ELISA with accuracy and quantitation,but also specialities of its own with simplicity and rapidity,so it fits routine and emergent use in the clinical labs very much.