A Review on Analytic Techniques of Fibrinolytic Protein
Ye Yun Huang Ming-xing · Zhongguo shengwu gongcheng zazhi · 2011
PAs are serine proteases of tryptic specificity which can convert the proenzyme,plasminogen,into an active enzyme plasmin.For the assay of PAs activity,four techniques have been established.Fibrin plate method and colorimetric assay using chromogenic substrates are two early methods which can be used for semi-quantitative analysis.Reverse fibrin autography and fibrin zymography are two new qualitative techniques which can be used to detect the molecular weight or isoelectric point of unknown PAs.The developing process and principle of these four techniques were reviewed,and the potential applications,advantages and disadvantages were discussed.