Analysis on Molecular Systematics of Chironomus rDNA in Water Treatment Plants
Zong Zu-sheng, Jinsong Zhang · China Water & Wastewater · 2005
Based on the traditional morphological classification, a specific amplifying primer was designed by searching homologous sequence of Chironomus rDNA in GenBank. The internal transcriptional spacing sequence (ITS1) of Chironomus rDNA in four water treatment plants was amplified and analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technology; meanwhile ITS1 region of Chironomus was measured by DNA sequence. And then the molecular phylogenetic tree was established for Chironomus based on the difference in sequence of ITS1 region. The result shows that ITS1 of Chironomus can be used as ideal molecule for characterizing Chironomus species.