Analysis of the platelet counting results in different testing mode for EDTA-dependent pseudothrombocytopenia
Zhu Hua · Clinical Medicine · 2013
Objective To check the platelet counting results in manual and dilution mode by hematology analyzer for EDTA-dependent pseudothrombocytopenia,provide basis to the methods of laboratory examinations.Methods Blood samples tested respctively by hematology analyzer in whole blood mode and dilution mode and manual performed with ammonium oxalate.Rechecked platelet counts by microscope.All testing regulations were compliance of Clinical Laboratory Improvement Amendments of 1988(CLIA'88).Results The platelet counts were nearly consistence at fifth minute.Platelet counts were more deviations at others time periods in whole blood mode and dilution mode.In dilution mode,the value of counting were relatively constant with the time prolonging.Because the platelet distributed,aggregated,dissociated in sixty minutes that led to nearly consistence of count value at the sixtieth and the fifth minute.Testing results were less changed performed with ammonium oxalate.Conclusions Complete the testing in five minutes in the dilution mode is preferred to rechecking platelet count.The cost is low and the result is reliable.