Construction of Subclone Library of Dragline and the Determination of Its Partial Sequence
Songping Liang · Journal of Nanhua University · 2004
Objective To obtain the whole length gene of dragline.Methods The Scos-DS1 DNA was digested with Hinf I and the subclone library of dragline was constructed using pUC19 as the vecter .After screening the library, 100 clones with insert around 500 bp in size were taken to determine the sequence.Results The subclone library of dragline gene connected by enzymolytic fragment of HinfI was succesfully constructed and its partial sequences were gained. Conclusion The whole length gene of dragline can be gained by using subclone method.