Effect of Artemisinin on Caspase-3 and Cytochrome c of Leuvemia Cell k562
HU Wen-liang · Zhonghua zhongyiyao xuekan · 2008
Objective:To investigate the effect of artemisinin the expression of Caspase-3 and cytochrome c in leukaemia cells k562 also to explore its possible mechanism of anticancer effects.Methods:K562 cells were cultured in vitro.The cell growth curve was drawn according to cell counts.Fluorescence microscopy was applied to demonstrate the presence of apoptosis.The expressins of Caspase-3 were assayed with reverse transcription-polymerase chain reaction(RT-PCR).The mitochondriaon and cytoplasm of K562 cells treated before and after action by artemisinin were separated and the expression of Cyt c was measured by Western blot.Results:When the concentrations of artemisinin were 1×10-4,1×10-5 and 1×10-6mol/L,the growth of cells was inhibited remarkably in a dose-dependent manner.Fluorescence staining showed obvious apoptosis,such as karyopyknosis and agglutination.RT-PCR assay shows the expression of Caspase-3.Artemisinin could lead to the decrease of mitochondrial cytochrome c concentration,which in turn lead to the increase of cytoplasmic cytochrome c concentration.Conclusion:Artemisinin could inhibit proliferation and induce apoptosis in K562 induction of delivery of cyt-c and activation of Caspase-3 may contribute to its effect.