Construction of Plant Expression Vector Harboring ThIPK2
R China · Shandong Agricultural Sciences · 2013
In order to get stress tolerant transgenic wheat plants without selective markers,the ThIPK2 gene sequence was got from the NCBI database,the gene codons were optimized based on wheat preference codons,the repeated and unnecessary restriction sites were removed,and the gene was synthesized. The synthesized ThIPK2 gene was constructed into vector with Ubiquitin promoter and AtSac66 terminator. The whole cassete was then ligated into a plant expression vector harboring Ac/Ds transposon,which could be further used for wheat transformation. Identified by restriction endonuclease,the expression vector was successfully constructed.