Optimization of SSR reaction system of Ephedra
Niu Jun-yi · Gansu Nongye Daxue xuebao · 2009
Ephedra intermedia was used in this study to optimize the several factors applied in SSR technique system.Optimized Simple Sequence Repeats(SSR) system was established as Mg2+ 1.5 mmol·L-1,dNTPs 0.5 mmol·L-1,Taq polymerase 1.0 U,primer 0.6 μmol·L-1.Optimized annealing temperature was 53 ℃ to 54 ℃.