Experemental Study of Changes of CH_(50) in Vitro Serum Culture Caused by Static Contact with Biomaterial
LI Liangzhon, Luke Qi Jiang · Beijing shengwu yixue gongcheng · 2001
Changes of total complement levels in surum can reflect immune response caused by antigen antibody reaction. Eight biomaterials were used to contact respectively with normal human serum for diffrent lengths of time to observe total hemolytic complement levels(CH 50 ). Immune responses of biomaterials were studied with standard methods. The result showed that the value of CH 50 measured often these materials were in vitro serum culture, was lower than that of the control group. But the decreases are different for different materials. The decreases of CH 50 of normal serum after being contracted with CH and PETF were less than those of other materials by 40 units. For two kinds of materials of PETF and PES, it decreased as length of time period increased (30,90 and 120 min). The longer the time contact with PETF of PES was the lower the CH 50 value of normal serum was. The results in the study suggest the CA and PETF materials can improve complement levels, in serum, can decrease CH 50 value, and can increase immune response.