Observation of the stability of ACD-EDTA anticoagulated blood in refrigeration for complete blood cell counts
Zhang Jin-fen · Medical Laboratory Sciences · 2004
Objective To study the stability of ACD EDTA anticoagulated blood in refrigeration, which could act as a kind of quality control material or calibrator for complete blood cell counts. Methods Adequate EDTA K 2 anticoagulant was added into ACD anticoagulated blood. The vials were stored in refrigerator for 10 days. The WBC, RBC, HGB, HCT and PLT were measured on the automated hematology analyzers including Beckman Coulter ACT diff Ⅱ, Sysmex K 4500, Abbott Cell Dyn 1200 and ABX Micros 60. Results The bias from the 5 parameters of complete blood cell counts measured on the 4 different instruments had no difference within 10 consecutive days, when each vial of ACD EDTA anticoagulated blood was opened and used on the same day. But the differences of bias from the parameters of WBC and PLT were significant( P 0.01), when the vial of ACD EDTA anticoagulated whole blood was open on the first day for measuring the parameters within 10 days in succession. Conclusions The stability of ACD EDTA anticoagulated whole blood might be stable in approximately 10 days, but the designated values are different on the 4 different analyzers. The preparation of ACD EDTA anticoagulated whole blood is not complicate. It could be used as a kind of quality control material or calibrator for complete blood cell counts.