Induction of oxidative damage by diclofenac in the mouse liver
Xue Li · Weisheng dulixue zazhi · 2004
Objective To evaluate oxidative stress involving metallothionein (MT) induced by different doses of diclofenac in mouse liver.Methods Adult male ICR mice were administered different doses of diclofenac (50,100,200,300 mg/kg) by oral gavage.Lipid peroxidation, glutathione (GSH) and MT contents in liver were determined.Results Malondialdehyde (MDA) in liver significantely increased in the dose\|dependent manner and time\|dependent manner.The highest of group 300 mg/kg was 1.9 fold compared to the control.GSH in liver rapidly decreased,the lowest was only 34% of he control.Moreover,GSH also showed preferable dose\|effect and time\|effect relationship.Liver MT content was markedly increased,the highest of group 50 mg/kg was 3.9 fold compared to the control.The MDA level had a negative correlation with the level of MT.Discussion Diclofenac can induce oxidative stress in mouse liver and MT may be one of the mechanisms that can protect this injury.