The preparation of C-reactive protein quality control material and related points for attention
Jing Yang · Laboratory Medicine · 2007
Objective To Prepare C-reactive protein(CRP) quality control material in concordance with international standard certified reference material(CRM)470 reference and used for various CRP quantitative diagnostic kit. MethodsThe stability at 37 ℃ of different ingredient for CRP quality control material was determined by reliable CRP quantitative diagnostic kit which must be consistent with international standard CRM470 CRP reference. ResultsLiquid CRP quality control material prepared in human or calf serum had a good stability,20 mg/L CRP had excellent stability for a month at 37 ℃,but at the same time the stability of high concentration CRP(100 mg/L) was not so stable.In the presence of ethylenediarmine tetraacetic acid(EDTA),the quantitative value would decrease immediately. ConclusionsThe liquid CRP quality control material is more stable than the freeze-dried CRP materials and the human or calf serum is the best medium for the preparation of CRP quality control material.EDTA can not be used in CRP quality control material.