Cloning and Expression Analysis of Copper/Zinc Superoxide Dismutase Gene From Antheraea pernyi
Yao Li · Canye kexue · 2009
Superoxide dismutase(SOD) is an important antioxygenic enzyme in living organisms.The open reading frame(ORF) sequence of Antheraea pernyi copper/zinc superoxide dismutase gene was obtained through RT-PCR method.Sequence analysis indicated that this partial cDNA sequence encodes a 154 amino acid polypeptide containing the conserved domain of Cu/Zn-SODs.The evolutionary analysis showed that its sequence identities with Bombyx mori,Hyphantria cunea,Bombyx mandarina and Drosophila melanogaster are 81.5%,81.7%,81.5% and 66.7%,respectively.The transcription activity of Cu/Zn-SOD gene in fat body,which was determined by RT-PCR,increased after 5 min of UV radiation,reaching the peak at 10 min and decreased at 15 min after UV treatment.