Phylogenetic relationships among several genera in Drosophilidae deducedfrom mitochondrial cytochrome b gene partial sequence
Yang Yong · Journal of Hubei University · 2004
By directly sequencing method of the PCR products the partial nucleotide sequences of mitochondrial cytb gene for five species or six isofemale lines belonged to Genus Drosophila (It included Subgenus Dorsilopha,Subgenus Drosophila and Subgenus Sophophora), Genus Phorticella, Genus Scaptomyza and Genus Scaptodrosophila in Drosophilidae were acquired.Reconstructed phylogenetic trees using maximum likelihood, neighbor joining and Bayesian methods.The research indicated that:in all choosen genera and subgenera,Genus Scaptodrosophila diverged at first and the second was Subgenus Dorsilopha; Genus Scaptomyza and Subgenus Drosophila had close relations and formed a paraphyletic group ,Genus Phorticella and that group were paraphyletic; Subgenus Sophophora diverged before Subgenus Drosophila,and the clade constituted by the representative species of Subgenus Sophophora was the sister group to the clade formed by Subgenus Drosophila and other genera (Genus Phorticella and Genus Scaptomyza).Also indicated that mitochondrial cytochrome b gene can be applied to the research work of the phylogenetic relationships at high level in Drosophilidae as an effective molecular marker.