DNA Extraction Optimization and Establishment of SSAP on Waxy Corn
Ke Duan · Xibei nongye xuebao · 2008
Base on the existed DNA extraction methods and the nature of waxy corn,waxy corn genome DNA extraction method has been optimized and improved.A260/280 of the DNA obtained by the method is between 1.8 and 2.0,and A260/230 is more than 2.0.The test result of agarose gel electrophoresis have proved that the DNA is compiete.it is more suitable for sequencespecific amplification polymorphisms(SSAP) analysis.Through comparative experiments,such as the combination of different enzymes restriction,different primers combination,SSAP analysis has been established on waxy corn,Clear bands and high polymorphisms have been got.The research made a foundation of germplasm,genetic diversity and fine breeding for waxy corn.