Application of yeast one hybrid system in plant gene engineering
Q Wang · Beijing Linye Daxue xuebao · 2008
The yeast one hybrid system is the new type system deriving from yeast two hybrid system to study the interactions between DNA and protein. This paper briefly introduces the principle and technical line of this system,and particularly summarizes the progress of this system in plant genetic engineering field,such as cloning the transcription factor genes of osmosis resistance type,of embryogenesis type,and of disease resistance type; confirming the interaction between known DNA and protein; making sure the localization of the confirmed DNA binding domain; moreover validating the transcriptional activation activity. Furthermore,the authors analyzed the problems existing in this system on the research of plant genetic engineering,as well as forecasted the development prospect according to the research.