Argument for effect of Vitamin K_3 applying to fungal counting with MTT assay
Xu Wang · Zhongguo linchuang yaolixue yu zhiliaoxue · 2005
AIM: To determine the concentration of vitamin K_3(VK_3) in fungal counting with MTT assay. METHODS: Microplate and MTT colorimetric assay were used to measure the effect of different VK_3 concentrations on fungal counting. RESULTS: The OD(optical density)value was affected by fungal and VK_3 concentration, measure wavelengh, and solvent addition-time at the same time. Generally speaking, the OD value was increased with fungal and VK_3 concentration increased. At a higher fungal concentration, there was little impact from solvent addition-time and remarkably impact from measure wavelengh. CONCLUSION: The reliable and stable results will be acquired in the conditions as follows: 106 fungal concentration, 0.1 mol·L -1 VK_3 concentration, and 570 nm measure wavelengh.