The effects of Shexiangbaoxin pills on the expression of cardiac angiotesin receptors in rat hearts with heart failure
LV Yang · Journal of Cardiovascular and Pulmonary Diseases · 2011
Objective:To investigate the effect of Shexiangbaoxin Pills(SXBXP) on plasma renin activ-ity(PRA) and angiotensin Ⅱ(AngⅡ) plasma levels of chronic heart failure(CHF) rats,and on expression of Angiotesin ⅡType 1 Receptor(AT1R) and Angiotesin ⅡType 2 Receptor(AT2R) in the messenger RNA(mR-NA) levels of non-infarction left ventricular tissue from rats with CHF induced by myocardial infarction.Meth-ods:Models of CHF established by left anterior descending coronary artery ligature.Fifty four Wistar rats were randomly divided into five groups: normal control group(group A),sham operation group(group B),CHF model group(group C),positive medicine control group(group D),small dose SXBXP group(group E) andlarge dose SXBXP group(group F),deployed intragastrically.Cardiac function was examined by echocardio-graphy before and after therapy,PRA and Ang Ⅱ plasma levels were tested by radio-immunity,mRNA ex-pressed levels were measured by semiquantitative reverse transcription polymerase chain reaction(RT-PCR) for AT1R and AT2R in non-infarction left ventricular tissue.Results:(1) There was no significant difference in left ventricular ejection fraction(LVEF) between groups A and B.Compared with group B,LVEF of groups C,D,E,F were significantly decreased(P 0.01) before therapy.After therapy,compared with group C,LVEF of group F was significantly improved(P 0.05).LVEF of groups D and E increased even though there was no significant difference were detected compared with group C.(2)Plama levels of PRA,AngⅡ,and mRNA expression levels of myocardial AT1R,AT2R in groups A and B appeared no diference(P 0.05).Compared with group B,PRA,AngⅡand AT2R expressed levels were markedly increased(P 0.01),AT1R were signif-icantly decreased(P 0.01) in group C.Compared with group C,levels of PRA and AT2R were significantly declined in groups D,E and F(P 0.05 and P 0.01).AngⅡ was markedly declined(P 0.05) and AT1R was significantly increased(P 0.01) in groups D and F.Conclusion: After SXBXP treatment,LVEF is in-creased and cardiac function is significantly ameliorated in rats with CHF.The therapeutical effect of SXBXP may be related to better blood supply for myocardium and down-regulation of PRA and AngⅡplasma levels,and regulation of AT1R and AT2R inclined to normal.The results show that that SXBXP can be used in treatment of CHF and the therapeutical effect of large dose SXBXP is superior to small dose SXBXP.