Study on the Effect of Bcl-2 in Deltamethrin Induced Neural Cells Apoptosis
WU You-tong · Carcinogenesis,Teratogenesis and Mutagenesis · 2004
BACKGROUNDAIM: To study the Influence of deltamethrin on the apoptotic rate and Bcl_2expression of neural cells in rat. MATERIAL AND METHODS: Male Wistar rats were randomly divided into control group and four deltamethrin treatment groups at5h、24h、48h and5days after treatment of12.5mg/kg deltamethrin intraperiˉtoneally.Apoptotic rate and Bcl_2expression were measured by FACS420Flow Cytometer;immunoblot was used to detect the expression of Bcl_2. RESULTS: Apoptotic rates in rat exposed to DM for24h、48h and5d(Hippocampus:8.45±1.02、9.44±1.14、7.58±0.75;Cerebral cortex:7.90±0.49、8.01±0.87、7.97±0.41)were higher than those of the control(Hippocampus:2.97±0.36;Cerebral cortex:3.50±0.48);the expression of Bcl_2of5h、24h、48h of hippocampus(5.27±0.33、4.72±0.13、5.80±0.86)and24h group of cerebral cortex(cerebral cortex:4.27±0.48)decreased apparently comparing with that of the control group(Hippocampus:7.16±0.61;Cerebral cortex:6.99±0.66). CONCLUSION: DM can interfere with the apoptotic rate and Bcl-2expression in rat brain.