Non-Covalent Complexes of the Antidepressant Compound SIPI5358 with Cyclodextrins
Xiaolei He · Acta Physico-Chimica Sinica · 2010
Non-covalent complexes of α-,β-,and γ-cyclodextrin(CD) with the antidepressant compound SIPI5358 were investigated by electrospray ionization mass spectrometry(ESI-MS),tandem mass spectrometry(MS/MS),UV spectroscopy,and fluorescence spectroscopy.The ESI-MS experimental results revealed that SIPI5358 reacted with the α-,β-,and γ-CD to form complexes with different coordination numbers.The tandem mass spectra further confirmed the formation of non-covalent complexes of SIPI5358 with β-CD.Binding of the complexes was also confirmed by UV as well as fluorescence spectra.The formation constant Kf was determined by fluorescence spectroscopy to be 3.45×103 mol.L-1 for the complex of SIPI5358 and β-CD.