Growth inhibitory effects of methyl jasmonate on human gastric cancer cell line SGC7901 invitro
Jianfu Zhang · Zhonghua zhongliu fangzhi zazhi · 2013
OBJECTIVE: To explore the growth inhibitory effects of methyl jasmonate(MJ) on human gastric cancer SGC7901 cell line,and investigate its mechanisms.METHODS: The MTT colorimetry was used to detect the growth inhibitory rates of MJ on human gastric cancer SGC7901 cell;Hoechst 33258 fluorochrome staining was employed to observe cell apoptosis;the apoptosis potential of cells was assessed by flow cytometry with Annexin Ⅴ-FITC/PI.The expressions Caspase-3 and Caspase-9 were detected by Western blott.RESULTS:MJ inhibited the growth of SGC7901 cells in a dose-and time-dependent manner.After exposed to 0.5,1.0 and 2.0 mmol/L MJ for 6 h,the inhibition rates were about(7.10±0.84)%,(18.22±1.82)% and(26.89±1.56)% respectively,compared with the solvent control group(0.43±0.26)% and DDP group(10.44±1.17)%,there was a statistically significant difference on total(F=232,P=0.000) and each group(P0.05);With the same dose MJ for 12h,the inhibition rates were about(12.44±1.20) %,(32.70± 1.29) %and(52.94±2.29) % respectively.Compared with the solvent control group(0.31±0.41) % and DDP group(22.60±1.97) %,there was a statistically significant difference on total(F=1 013,P=0.000) and each group(P 0.01);for 24h,the inhibition rates were about(17.37±1.95) %,(41.79±4.40) % and(71.93±4.65) %,respectively.Compared with the solvent control group(0.34±0.21) % and DDP group(27.72±2.45) %,there was a statistically significant difference on total(F=444,P=0.000) and each group(P0.01).2mol / L MJ after 24hhad the best inhibition rates.Hoechst 33258fluorochrome staining showed that there were many apoptotic cells in MJ group.The apoptosis rate was(68.14±7.91) %,after exposed to 2.0mmol / L MJ for 24h,compared with the solvent control group(6.98±1.45) %(P=0.013).The expressions of Caspase-3and Caspase-9protein were increased.Above-mentioned inhibitory effects of MJ on SGC7901cell were abolished,respectively by the pretreatment with capsazepine,an antagonist of vanilloid receptor subtype 1.The percentage of inhibition rate and apoptosis dropped to(38.79±2.77) %and(39.81±4.57) %(P=0.05;P=0.045),respectively.The expressions of protein were also decreased.CONCLUSIONS : MJ can significantly inhibit the growth of SGC7901cells through inducing cell apoptosis.Its molecular mechanism was probably associated with vaniloid receptor subtype 1(VR1) signal pathway.