The Affected Factors on Separating Red Blood Cell Numbers by Discomtiunous Density Gradient Separation
Deqin Wang · Journal of Clinical Transfusion and Laboratory Medicine · 2011
Objective To analyse the affected factors on separating whole blood by discontinuous density gradient separation,and its effect on the separation.Method To use orthogonal design to analyse separation outcome on different separation conditions(centrifugal force,centrifugal time,centrifugal temperature),and to apply multivariate linear regression analysis to analyse effects on separation of different individual parameters(red blood cell concentration,mean corpuscular volume,individual age).Results Data indicated that the centrifugal force,centrifugal time,specimen concentration,mean corpuscular volume,and individual age influenced the results,among which mean corpuscular volume was dominant.Conclusion Different separation conditions as well as the same separation conditions of different specimens,their separation outcome were difference.If the factors controlled manual and choiced selectively,you could get a relatively stable separation result.