The performance evaluation of platelet counting in Sysmex XE-5000
Xiaomin Liu · Laboratory Medicine and Clinic · 2011
Objective To evaluate the platelet(PLT) counting performance quality of Sysmex XE-5000 automatic hematology analyzer.Methods 257 EDTA-K2 blood samples were counted by XE-5000 with electric impedance(PLT-I).According to PLT histogram,MPV and MCV,we separated the results to normal platelet,large platelet and microcytes from different PLT shape,and used electric impedance(PLT-I) and microscopic(PLT-M) methods to compare with them.Another blood samples from 60 healthy people were tested with RBC fragment interference experiment.All data were analyzed by SPSS 12.0 and Excel.Results There were significant correlations in the results among the three methods in normal PLT.In large platelet group,PLT-I pseudo reduced.PLT-I and PLT-M method results were statistically significant and PLT-O with the PLT-M method showed no significant difference.Microcytes group increased false in PLT-I.PLT-I and PLT-M method results were statistically significant.The result showed that the PLT-O method had great ability of anti-interference in RBC fragment interference experiment.Conclusion Sysmex XE-5000 automatic hematology analyzer is of high accuracy for normal PLT.PLT-O and PLT-M are better than PLT-I in counting abnormal PLT which PLT histogram,MPV,MCV are unusual.