Soil-based Eco-toxicity of Diesel Oil Contamination to Seed Germination and Seedling Growth of Wheat
Zhou Qi-xing · Nongye huanjing kexue xuebao · 2006
Effects of diesel oil stress with different concentrations on seed germination, seedling growth of wheat (Triticum aestivum L.) and the activities of antioxidant enzymes in the leaf and root of wheat seedlings under artificially controlled conditions in laboratory were studied. The results showed that the seed germination rates were not significantly inhibited in the treatments of 500 to 15 000mg·kg-1 diesel addition except the 30 000 mg·kg-1 treatment in which the germination rate fell to 61.7%. Root length and shoot length were markedly inhibited under the diesel stress even in the lower concentration treatment with 500 mg·kg-1. Sensitivities of emergency ratio, root length and shoot length followed the order: root lengthshoot lengthemergency ratio. The seedling growth was stimulated in the lower diesel concentration of 500 mg·kg-1, while inhibited in treatments with diesel concentration of 1 000~30 000 mg·kg-1 in which contents of chlorophyll a, seedling height and fresh weight decreased in some degree and the more the diesel stress increased, the more the damage increased. The antioxidant enzymes in the root and leaf of wheat seedlings responded to the diesel stress with the increased concentration and prolonged duration, the activity of superoxide dismutase (SOD) increased with the diesel concentration increasing, but the activity of peroxidase (POD) and catalase (CAT) in the leaf and root of wheat seedlings decreased. The content of malonyldialdehyde (MDA) in the root of wheat seedling differed from that in the leaf under diesel stress.