Spectrophotometric Study on the Reaction of Carboxynitroazo and Serum Proteins
Hu Qiu · Huaxue Shijie (Chemical World) · 2000
It was found that red carboxynitroazo can react with serum proteins forming a blue violet complex in Britton Robinson buffer at pH 3.0 and room temperature, which gives maximum absorption peak at 680 nm with 140 nm red shift in from that of carboxynitroazo. A molar absorptivity of 2.60×10 5 L/mol·cm (BSA) and Lineary range of 20~140 μg/mL for proteins were determined. Standard curves were constructed under optimum conditions obtained in our lab. The Lineary range is wide. This assay is sensitive, accurate and is tole rant of many foreign substances. Moreover, the reagents are stable and this method is easy to carry out.