The Preliminary Study on Effects of Hyperthermia on Reversion of Multidrug Resistance of Human Gastric Cancer Cell Line SGC7901/ADM
Chunhua Li · Xiandai shengwu yixue jinzhan · 2009
Objective:To investigate the reversal effect of hyperthermia on multidrug resistance of SGC7901/ADM cell line. Methods:Drug resistant human gastric cancer cell line SGC7901/ADM and sensitive human gastric cancer cell line SGC7901 has been selected.MTT test was employed to detect the cell survival rate and IC50 in the different temperatures and different drugs.Results:It was found that drug resistant human gastric cancer cell line SGC7901/ADM showed not only resistance to the inductive ADM but also cross resistance to CDDP,5-FU and TAX.High temperature of 43℃could lower the survival rate of SGC7901/ADM cell and resistance index (RI)significantly.Reversal multiple to chemical drugs like ADM,CDDP,TAX,5-FU is respectively 3.77,2.24,6.25 and 1.11. Conclusions:43℃hyperthermia could effectively enhance the sensitivity of SGC7901/ADM cell to chemical drugs like ADM,CDDP, TAX and partially reverse the multidrug resistance.It is suggested that hyperthermia could be used to overcome multidrug resistance.