Exploration on Induction of Breast Cancer Bcap37 Apoptosis by Oridonin
Xiumei Zhou · Journal of Zhejiang Sci-Tech University · 2013
This paper discusses the role of oridonin in the proliferation and apoptosis of breast cancer cell Bcap-37 and the mechanism causing apoptosis;treats Bcap-37 cell with Ori of different concentrations;detects cell growth inhibition rate with MTT method;detects apoptosis by using flow cytometry and the mechanism of apoptosis with Western blot.The result is that 48~96 μmol/L Ori can significantly inhibit the proliferation of Bcap-37 cell(P0.05).After 24 h of operation of 48 μmol/L Ori,the proliferation inhibition rate reaches 40%,while 96 μmol/L Ori only needs 12 h treatment and the proliferation inhibition rate reaches 50%.After 24-hour treatment of 96 μmol/L Ori,the apoptosis rate of Bcap-37 cell is 21.6%.Western blot detects up-regulation of Fas,release of cytochrome c,activation of Caspase 9 and shear of its downstream signaling protein Caspase3 and its substrates PARP and ICAD.It further detects the down-regulation of Survivin and up-regulation of GADD34.Based on these experimental results,it is inferred that the induction of apoptosis of breast cancer cell Bcap37 by oridonin as a potential anti-breast cancer preparation might be related to Survivin and GADD34.