Experimental Study on Two Old Drugs Decoction on the Model of Subacute Induce-aging in Mice
Yong Liu · Journal of Liaoning College of Traditional Chinese Medicine · 2003
Objective: To explore the mechanism of two old drugs decoction which can negulat qi to resolve and dissolve phlegm and resist Methods: Use the subacute aging model in mice with D gal.SC the neck and back of mice by D gal(120mg/kg) for 40days,SC the herbs group by D gal let them drink the concentrated two old drugs decoction for 40 dyas,and compared with the empty group,than observe the effect on the indices of the brain,dthymus,spleen,liver and kidney,the activity of SOD of serum,MDA and the ultrastructure of macrophage of the peptidergic neurons. Result: The indices of the subacte aging model in mice with D gal such as brain,thymus,splcen,liver and kidney discrease,the activity of SOD,discreases obviously ( P 0 01),MDA increase obviously( P 0 01),the mitochondria of peptidergic neurons tumid,the cristae cut off,megamitochondria,ER(endoplasmic reticulum) tumid,megamitochondria,nuclear envelope blurred or perinuclear space enlarge,nuclear protein is destroyed,the nuclear matrix drop,nucleolus move away and cut off,the morphological change of the(Lipofucin)LPF,etc.two old drugs decoction can increase the indices of the brain,thymus of the modal mice,increase the actively of the SOD,decrease the MDA and the destroyed of the nucleolus organizer. Conclusions: Two old drugs decoction can resist geriatric change.Meanwhile these result provide reference data the theory of Phlegm related to geriatrics.