Apoptosis in Lewis carcinoma cell induced by euphorbia fischeriana steud and its mechanism
Tengda Zhao · Zhonghua zhongliu fangzhi zazhi · 2012
OBJECTIVE:To investigate the effect of euphorbia fischeriana steud(LDE)on apoptosis and expression of Caspase-9 mRNA in Lewis lung carcinoma.METHODS:Lewis cells were exposed with LDE of different concentration(0.2,0.4 and 0.8 mg/mL) for 24 hours.Annexin-Ⅴ/PI double label staining was applied to detect the change of apoptosis rate.Flow cytometry was applied to detect the change of cell cycle.The mRNA level of Caspase-9 was detected by Real-time PCR.RESULTS:By Annexin-Ⅴ/PI double label staining to examine apoptosis of control and low,middle and high LDE dose group(0.2,0.4 and 0.8 mg/mL),and the rate respectively was(0.331±0.012)%,(8.27±0.067)%,(28.19±0.270)%,(32.96±0.14)%.Flow cytometry showed that the G0/G1 phase rate of the control and low,middle and high LDE dose group were(50.3±0.77)%,(44.2±1.82)%,(34.1±1.56)%,(25±0.72)%.Real-time PCR showed that Caspase-9 mRNA expression level of the control and LDE group were 1.00 and 1.64.CONCLUSION:LDE can effectively induce apoptosis of Lewis cells through blocking the cell cycle at G0/G1 phase and increase the expression of Caspase-9 mRNA level.