Impact of MCV on the PLT counting using different hematology analyzers
Zhixiong Zhang · Medical Journal of West China · 2005
Objective To probe into the effects of MCV on the PLT counting using Hematology Analyzers according to different principles.Methods Impedance method-floating threshold, impedance method-fitting curve, impedance method-sheath flow and two dimensional laser light scattering method were used to count 3 different groups of PLT(MCV≥80fl, 70≤MCV80fl, MCV70fl), and the results of these four methods were compared with those of reference method. Results Variance analysis showed that the five methods for PLT counting had not significant difference(P0.05) when MCV≥ 80 fl. If the MCV of samples was between 70 and 80 fl or less than 70 fl, there was a significant difference(P0.05) among these methods. The results by CD1700 or GEN,were significantly different(P0.05) from other three methods, but there was no significant difference(P0.05) among ADVIA120, XE2100 and reference methods.Conclusion The two-dimensional laser light scattering method is superior to the impedance method when the abnormal sample with small red blood cells was detected.