Study on the reduction of blood platelet caused by anticoagulant EDTA-K_2
Liu Si-jing · Laboratory Medicine and Clinic · 2011
Objective To discuss the related process and the reason about the reduction of blood platelet caused by anticoagulant EDTA-K2.Methods By using automated hematology analyzer-Sysmex XE2100,we measured the value of WBC and PLT that from the samples which used anticoagulant sodium citrate and EDTA-K2 separately.Then we compared and statistically analyzed the results that from the dilution model of sysmex KX-21 and manual scope checking.We compared the value of WBC and PLT that from two kind of anticoagulant specimens in 5,30 and 60 min.Then we made smears with anticoagulant and no anticoagulant separately,and observed the changes in number and morphology of PLT.Results Compared the value of WBC and PLT from EDTA-K2 anticoagulant with that from diluted model KX-21 and the results of microscopic,the difference was significantly different(P0.01).the difference of the value of WBC and PLT from EDTA-K2 anticoagulant in 5,30,60 min was significantly different(P0.01).Compared with those without anticoagulation,EDTA-K2 anticoagulant smear had obviously been a lot of blood smears PLT aggregation,and satellite phenomenon.Conclusion EDTA-K2 can promote PLT aggregation and cause the reduction of PLT,and the process takes place within 5 min,and complete within 30 min.