Quantitative and Qualitative Mass Spectrometric Analysis of Anticancer Agents, Drugs of Abuse and Enzyme-Inhibitor Complexes
Kerri M. Smith · OhioLink ETD Center (Ohio Library and Information Network) · 2012
Mass spectrometry is a valuable tool in the analysis of many types of compounds.From small molecules to large proteins, mass spectrometry can help to interrogate samples and give insights to the origins of disease as well as calculate an exact concentration.Bioanalytical method development is an integral component in the measurement of such compounds.To facilitate proper analytical investigation, the process of method development, analysis, and interpretation must be understood.This work describes the basis and procedure for bioanalytical method development and its detailed application to preclinical studies of the antineoplastic agent hexamethylene bisacetamide and measurement of illicit drugs of abuse benzylpiperazine and trifluoromethylphenyl piperazine using liquid chromatography tandem mass spectrometry.Additionally, procedures for analyzing protein-drug interactions and their implication in antibiotic resistance are discussed.