The wwPDB common tool for deposition and annotation
Martha Quesada, J. Westbrook, Tom Oldfield, J. Young, Jose Swaminathan, Z. Feng, S. Velankar, T. Matsuura, Eldon L. Ulrich, S. Madding, Gerard J. Kleywegt, John L. Markley, Haruki Nakamura, Helen Miriam Berman · Acta Crystallographica Section A Foundations of Crystallography · 2011
Poster Sessions C403 buffer (PB) (pH 7.0) containing 0.1 M NaCl.Solution SAXS measurements were carried out at the BL45XU RIKEN Structural Biology Beamline I (wavelength, λ = 0.09 nm) of the SPring-8, Harima, Japan.Two-dimensional (2D) SAXS images were recorded with a CCD camera.For aggregation experiments, the complementary DNA and then NaCl were added to the suspension in such a way that the final concentrations of complementary DNA and NaCl were 0.5 µM and 1 M, respectively.The non-crosslinked assembly has a crystalline structural order but less long-range ordering.The inter-surface distance between the particles increased with the length of dsDNA.From the experiments using the binary mixtures, it was unveiled that the DNA-functionalized particles with the same core size assemble together irrespective of tethered DNA.Furthermore, it was demonstrated that, for smaller core sizes or longer DNA lengths, their nanoparticles covered with full-matched dsDNA can disperse stably above a temperature which is lower than the melting temperature of the dsDNA.This may cause by steric stabilization of DNA molecules.It is considered that the attractive interaction in the non-crosslinking aggregation is attributable to van der Waals potential between core particles, and the contribution of the end-to-end stacking attraction between dsDNA might be little.As a plausible mechanism, the feature of non-crosslinking aggregation interaction could be characterized by steric repulsion stabilization due to entropic loss in conformation and mobility of DNA molecules.