MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) Colorimetric Assay for Mitogen-induced Activation of Chicken Peripheral Blood Lymphocytes
Yasuhiro Kondo, Tomonori AOKI, Asaki ABE, Akira Tanabe · Nihon Chikusan Gakkaiho · 1995
An MTT colorimetric assay was used to measure the mitogenic response of chicken peripheral blood lymphocytes (PBL) to T cell mitogens, Concanavalin A (Con A) and phytohemagglutinin (PHA). OD values obtained in T cell mitogen-stimulated PBL rose as the incubation times increased. The largest OD values (optimal concentrations) were observed at 0.1mg/ml of PHA concentration and at 31.25 to 62.5μg/ml of Con A concentrations, respectively. The results suggest that the mitogenic response of chicken PBL is maesurable by MTT colorimetric assay, although the data must be compared with values obtained by radioactive nucleotide incorporation assay.