Crystal structure of cytochrome P450 105A1 in complex with 1α,25-dihydroxyvitamin D3
H. Sugimoto, Raku Shinkyo, Kei Hayashi, Sachiyo Yoneda, M. Yamada, Masaki Kamakura, Shinichi Ikushiro, Yoshitugu Shiro, T. Sakaki · Acta Crystallographica Section A Foundations of Crystallography · 2008
Sessions 4inside surface is located at the D-pathway entrance.The zinc binding affinity for the second site suggests that the zinc site is tightly coupled with the proton-pumping site.Recently, we analyzed Zn/Cd-binding to monomeric CcO which gives crystal packing different from that in the dimeric CcO crystal.The x-ray structural analysis showed Znbinding to the Zn2, Zn3 and additional sites including the site near the K-pathway entrance.Several Zn-binding sites have been found on the outside surface.However none of them is located on the subunit I surface from which pumping protons exit.