Prevalence and antibiogram of pseudomonas aeruginosa isolated from clinical specimens in a Teaching Hospital, Kathmandu
Sony Shrestha, Ritu Amatya, Ram Prasad Adhikari · International Journal of Infectious Diseases · 2016
Background: Pseudomonas aeruginosa is a leading cause of nosocomial infections. Increased resistance in this organism continues to pose a significant threat to patient care because of limited therapeutic options. Knowledge of the prevalence of P. aeruginosa in various infections and their antibiotic susceptibility pattern is of utmost importance for selection of appropriate therapy. Objective of the present study was to determine the prevalence and resistance pattern of P. aeruginosa isolated from various clinical specimens to Imipenem and other commonly used antibiotics in Nepal Medical College and Teaching Hospital (NMCTH), a teaching hospital in Kathmandu, Nepal. Methods & Materials: P. aeruginosa isolated from various clinical specimens like pus, sputum, blood, urine, catheter tips (devices), renal stones and body fluids processed in the clinical laboratory, department of Microbiology, NMCTH were included in the study. Isolation, Identification and antimicrobial susceptibility pattern were performed using standard microbiological techniques. Results: A total of 102 isolates of P. aeruginosa were evaluated. The prevalence rate of the organism was 5.1%, out of which 75 (73.5%) were from inpatients and 27 (26.5%) were from outpatients departments. Urine and sputum yielded highest number of the isolates 37 (36.3%) each followed by pus and devices 10 (9.8%) each. Highest percentage of the organism 36 (35.3%) was isolated from the patients who were of more than 60 years of age. Nineteen (18.6%) of the organims was seen to be multi-drug resistant. The organism showed maximum resistance to Piperacillin (57.1%) followed by Ciprofloxacin (36.7%), Ofloxacin (28.8%) and Gentamycin (30.9%). Only 6.5% of the isolates were resistant to Imipenem. Conclusion: The antibiotic susceptibility pattern of bacterial pathogen like P. aeruginosa in the hospital settings should be continuously monitored and the results readily made available to clinicians so as to maximize the possibility of administering an effective therapeutic agent whenever needed.