Defenition of quantitative content of dihydroquercetin in dietary supplements. Report 2
Олександр Юрійович Владимиров, Петро Овксентійович Безуглий, Ганна Олександрівна Бур’ян, Шарк Амжад Ібрагім Абу, Лідія Олександрівна Петрушова, Тетяна Вікторівна Алексєєва, Олександр Анатолійович Здорик, Василь Васильович Гриненко, Ірина Анатоліївна Данилова · ScienceRise · 2016
Prevention of cardiovascular disease is a vital measure for each person and for the entire nation. To prevent diseases of the cardiovascular system as a source of essential bioactive substances are used dietary supplements on the base of medicinal plants and bioflavonoids.Aim. The aim was to develop specific technique to quantify the dihydroquercetin in dietary supplements "Kapilliarol", "Kapilliarol forte" and "Cardio-Vert with Dihydroquercetin" and determination of its validation characteristics.Methods. Photocolorimetric method of quantification of DQW, which was based on the specific reaction of cyanidine chloride formation when added zinc powder to dihydroquercetin solution in an acidic medium.Results. Flavonoid contents recalculating on dihydroquercetin in dietary supplements in "Kapilliarol", "Kapilliarol forte" and "Cardio-Vert Dihydroquercetin of" has been stated, the basic validation characteristics have been determined. Contents of DQV has been defined in dietary supplements: "Kapilliarol" not less than 0.02 g, "Kapilliarol Forte" not less than 0,015 g, "Cardio-Vert with Dihydroquercetin" not less than 0.02 g.Conclusions. The obtained metrological data of the developed photocolorimetric technique has shown that the method reproduced in conditions of two different laboratories with confidence probability of 95 % of deviation of unit value was 100,93±2,15 %. Complete uncertainty of analysis was 1.35 %, including sample preparation uncertainty of 1.15 %, that indicates a significant influence of sample preparation on the results of the analysis