Expression, Purification and Characterisation of Haemophilus influenzae 3-Isopropylmalate Dehydrogenase (LeuB)
Sara Martignon, Franca Rossi, Menico Rizzi · Protein and Peptide Letters · 2007
3-isopropylmalate dehydrogenase (3IPMDH) is the third enzyme in leucine biosynthesis and a promising target for the development of broad-spectrum antibacterial agents. We report here the expression, purification and biochemical characterisation of Haemophilus influenzae 3-isopropylmalate dehydrogenase. The observed enzyme inhibition by the reaction product NADH could represent a regulatory mechanism for 3IPMDH. Keywords: Haemophilus influenzae, 3-isopropylmalate dehydrogenase, recombinant enzyme, leucine biosynthesis, antibacterial target, product inhibition