Padlock and RCA signature predication software
Shea N. Gardner, Thomas A Kuczmarski · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) · 2023
This software predicts DNA signatures compatible with padlock probe and rolling circle amplification (RCA) platforms. Specifically, the software takes a multiple sequence alignment, generates a consensus of conserved bases, and from these conserved regions selects forward and reverse primers that are immediately adjacent to one another, which is the desired orientation for assays such as padlock probes and RCA.