Effects of nonylphenol on L-type Ca2+ currents in isolated guinea-pig ventricular myocytes.
Qinghua Gao, Tong Zhu, Feng Jie Guo, Lei Yang, Meimi Zhao, Gang� Li, Liying Hao · Zhongguo Yike Daxue xuebao · 2010
Objective To observe the effects of nonylphenol(NP)on L-type Ca2+ currents(ICa-L)in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca2+ currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited ICa-L at different potentials.Nonylphenol(10-6 mol·L-1,10-5 mol·L-1)reduced the peak amplitude of ICa-L from-3.2±1.5 pA·pF-1 to-1.6±0.8 pA·pF-1(P 0.01)and-1.4±0.7 pA·pF-1(P 0.01),respectively.Nonylphenol did not influence the activation curve of ICa-L significantly.Conclusion Nonylphenol could inhibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner.